actinomyces oris sps 871  (ATCC)

Journal: Antibiotics

Article Title: Assessment of Oral Microbial Viability by 2,6-Dichlorophenolindophenol a Redox Agent

doi: 10.3390/antibiotics14060590

Figure Lengend Snippet: Relationship between viable cell counts and DCIP-reducing activities of oral organisms A. viscosus ( A ), A. actinomycetemcomitans ( B ) V. atypica ( C ), S. mutans ( D ) and C. albicans ( E ). For each organism, analyses indicate statistically significant correlations between DCIP results and corresponding viable counts ( p < 0.05).

Article Snippet: Oral bacteria Actinomyces viscosus (ATCC 19246), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Streptococcus mutans (ATCC 25175) were cultured in Brain Heart Infusion broth, while Veillonella atypica (ATCC 27215) in was cultured in Peptone Yeast Extract broth, at 37 °C, in an anaerobic chamber for 48 h. Prior to experiments, bacteria were centrifuged and resuspended in fresh medium to an optical density of 0.8 (for A. viscosus ) and 0.3 for the other strains at 600 nm.

Techniques:

Journal: Antibiotics

Article Title: Assessment of Oral Microbial Viability by 2,6-Dichlorophenolindophenol a Redox Agent

doi: 10.3390/antibiotics14060590

Figure Lengend Snippet: Effect of heat on DCIP reduction by cultures of A. viscosus. Bacterial cultures were incubated at the indicated temperature for the specified periods of time prior to the assays. Results are shown as means of triplicate samples ± standard deviation. In comparison to untreated controls, heat treatment of bacterial cultures at 50 °C, 60 °C or 70 °C demonstrated statistically significant reductions in DCIP results by t -tests ( p < 0.05). Symbol * identifies statistically significant differences for the evaluation from all other evaluations. Symbol † identifies statistically significant differences between the 60 °C treatments and from the 50 °C treatment. Symbol § identifies statistically significant differences between the 70 °C treatments and the 50 °C and 60 °C treatments irrespective of durations. There were no differences at 70 °C for any of the treatment durations.

Article Snippet: Oral bacteria Actinomyces viscosus (ATCC 19246), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Streptococcus mutans (ATCC 25175) were cultured in Brain Heart Infusion broth, while Veillonella atypica (ATCC 27215) in was cultured in Peptone Yeast Extract broth, at 37 °C, in an anaerobic chamber for 48 h. Prior to experiments, bacteria were centrifuged and resuspended in fresh medium to an optical density of 0.8 (for A. viscosus ) and 0.3 for the other strains at 600 nm.

Techniques: Incubation, Standard Deviation, Comparison

Journal: Antibiotics

Article Title: Assessment of Oral Microbial Viability by 2,6-Dichlorophenolindophenol a Redox Agent

doi: 10.3390/antibiotics14060590

Figure Lengend Snippet: The effect of mouthrinses on DCIP-reducing activity by A. viscosus. Bacteria were incubated for 20 min with indicated concentrations of mouthwashes formulated with cetylpyridinium chloride [CPC] or chlorhexidine [CHX] prior to the DCIP assay. In comparison to placebo-treated bacterial cultures, treatment of bacterial cultures with either the CPC or CHX mouthrinse demonstrated statistically significant reductions in DCIP results by t -tests ( p < 0.05).

Article Snippet: Oral bacteria Actinomyces viscosus (ATCC 19246), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Streptococcus mutans (ATCC 25175) were cultured in Brain Heart Infusion broth, while Veillonella atypica (ATCC 27215) in was cultured in Peptone Yeast Extract broth, at 37 °C, in an anaerobic chamber for 48 h. Prior to experiments, bacteria were centrifuged and resuspended in fresh medium to an optical density of 0.8 (for A. viscosus ) and 0.3 for the other strains at 600 nm.

Techniques: Activity Assay, Bacteria, Incubation, Comparison

Journal: Antibiotics

Article Title: Assessment of Oral Microbial Viability by 2,6-Dichlorophenolindophenol a Redox Agent

doi: 10.3390/antibiotics14060590

Figure Lengend Snippet: The effect of doxycycline on ( A ) DCIP-reducing activity and ( B ) growth of A. viscosus . Bacteria were cultured at 37 °C in anaerobic chamber for 24 h in the presence of antibiotic at the indicated concentrations. DCIP reduction assay was performed at the end of culture. The percentage change in OD at 595 nm at the end of culture relative to the start is a measure of growth. Results are shown as means of DCIP-reducing activity from triplicate samples ± standard deviation. In comparison to untreated controls, antibiotic treated bacterial cultures demonstrated statistically significant reductions in DCIP results by t -tests ( p < 0.05).

Article Snippet: Oral bacteria Actinomyces viscosus (ATCC 19246), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Streptococcus mutans (ATCC 25175) were cultured in Brain Heart Infusion broth, while Veillonella atypica (ATCC 27215) in was cultured in Peptone Yeast Extract broth, at 37 °C, in an anaerobic chamber for 48 h. Prior to experiments, bacteria were centrifuged and resuspended in fresh medium to an optical density of 0.8 (for A. viscosus ) and 0.3 for the other strains at 600 nm.

Techniques: Activity Assay, Bacteria, Cell Culture, Standard Deviation, Comparison

Journal: Antibiotics

Article Title: Assessment of Oral Microbial Viability by 2,6-Dichlorophenolindophenol a Redox Agent

doi: 10.3390/antibiotics14060590

Figure Lengend Snippet: The effect of amoxicillin on ( A ): DCIP-reducing activity, and ( B ): growth, of A. viscosus . Bacteria were cultured at 37 °C in anaerobic chamber for 24 h in the presence of antibiotic at the indicated concentrations. DCIP reduction assay was performed at the end of culture. Percent change in OD at 595 nm at the end of culture relative to the start is a measure of growth. Results are shown as means of DCIP-reducing activity from triplicate samples ± standard deviation. In comparison to untreated controls, antibiotic treated bacterial cultures demonstrated statistically significant reductions in DCIP results by t -tests ( p < 0.05).

Article Snippet: Oral bacteria Actinomyces viscosus (ATCC 19246), Aggregatibacter actinomycetemcomitans (ATCC 43718) and Streptococcus mutans (ATCC 25175) were cultured in Brain Heart Infusion broth, while Veillonella atypica (ATCC 27215) in was cultured in Peptone Yeast Extract broth, at 37 °C, in an anaerobic chamber for 48 h. Prior to experiments, bacteria were centrifuged and resuspended in fresh medium to an optical density of 0.8 (for A. viscosus ) and 0.3 for the other strains at 600 nm.

Techniques: Activity Assay, Bacteria, Cell Culture, Standard Deviation, Comparison